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Seeing Molecular Interactions of Large Complexes by Cryo Electron Microscopy at Atomic Resolution
Suppose one has never seen an automobile and is asked to perform the simple task—while blindfolded—of adding gasoline to the car and checking its oil. A task like this is impossible to perform until ...
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Automated Colony Counting: A Critical Requirement for Advanced Cancer Research
The colony counting method has long been recognized as the primary method for measuring the effects of radiation, chemotherapeutic drugs, and other agents on cell viability.
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Electronic Pipetting: The Call for Innovation
Take a close look around most laboratories and you will find an old, barely used fixture. No one knows who ordered it or who it belongs to. It is yellowed, dusty, and top heavy, with a small keyboard ...
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Improved Pipetting Technique: An “Out-of-the-Box” Solution to Reduce Manual Pipetting Errors
Handheld air-displacement pipets are used in virtually every laboratory around the world. The technique and skills of individual pipet operators are critical to the accuracy of results of liquid ...
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The Scales of Scientific Justice in Translational Science: Data Reproducibility and Verifiable Science
The traditional “scales of justice” represent truth and fairness. For the scientific community, the balance is between big data (terabytes of data across numerous databases) on one scale and ...
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LIMS-BPMS Coupling: A Novel Approach for Flexible End-to-End Workflow Automation in Life Science Laboratories, Part 1
Generalized life science automation (LSA) includes all application areas of laboratory automation, i.e., health care, pharmaceutical development, chemistry, and biology. The connection of all ...
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Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures
Traditional RNA detection methods, which analyze RNA levels in lysed, permeabilized, or fixed cell samples, make it challenging to understand the role of one gene in the context of a tissue, organ, or...
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Quality Control: An Important Success Factor in Nucleic Acid-Based Analysis
Quality control (QC) is becoming increasingly important in nucleic acid-based testing. As detection technologies become more and more sensitive, final results are influenced not only by biological ...
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Meeting the Challenge of Biological Changes Postsampling
Understanding the role of proteins, peptides, and their post-translational modifications, e.g., phosphorylations, in normal and diseased biological samples is crucial to elucidate in vivo biological ...
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Driving Scientific Innovation: Managing Biologics R&D Data Effectively
Over the past decade, the biological laboratory has become an increasingly digitally enhanced place. These days a skills section of a CV is more likely to include various instruments and software ...
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Book Review: The Physics of the Future
During the last year, I’ve been attracted to books describing paradigm shifts enabled by today’s new technology, particularly big data and IT—hence my interest in books from visionaries, which might ...
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A Matter of Speed: Real-Time PCR for the Rapid Detection of Mycoplasma Contamination
Mycoplasma are among the world’s smallest bacteria capable of independent reproduction. They belong to the class of Mollicutes and have a very slow and parasitic growth.
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Measuring Absolute Molar Mass Using UHPLC SEC-MALS
The characterization of aggregates or fragments in a biomolecular sample for applications such as bioanalytical chemistry and clinical diagnostics has traditionally been carried out using size ...
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Best Practices for the Use of Micropipets
Every day, air-displacement pipets are used to quantitatively dispense sample and reagent aliquots for reaction, routine analyses, and specialized tests.
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The Importance of an Integrated Informatics Solution for Life Science Organizations and Keys to Successful Deployment
The exorbitant cost of bringing a new pharmaceutical product to market in the U.S. is well documented, with estimates as high as 12 years and $11 billion. There are many factors that drive this cost, ...
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