Application Notes

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Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays
Friday, June 07, 2013
Amedeo Cappione Masaharu Mabuchi David Briggs Timothy Nadler
Many protein detection methods, including Western blotting, flow cytometry, ELISA, and immunohistochemistry, require the use of target-specific probes, such as antibodies, that are detected via two-... Read More
Development of a 100% Dimethyl Sulfoxide (DMSO) Sample Solution for Liquid Handler Performance Verification
Friday, June 07, 2013
Rachel Parshley John Thomas Bradshaw Karl Olson
Dimethyl sulfoxide (DMSO) has become one of the most important solvents used for high-throughput screening (HTS) and compound management applications due to its ability to readily dissolve a wide ... Read More
A Novel Sample Preparation Method for Complete Digestion of Complex Pharmaceutical Matrices
Friday, June 07, 2013
Reynhardt Klopper Eric Fox
For more than 100 years, the USP General Chapter <231> concomitant visual test was the standard method for the determination and quantification of heavy metal impurities present in pharmaceutical products ... Read More
Spectroscopic Ellipsometry Characterization of Thin Films Used in the Food Packaging Industry
Tuesday, June 11, 2013
Michelle N. Sestak
Process control and optimization is critical for low-cost, high-throughput, thin-film applications, such as barrier layers used in the food packaging industry. One useful technique for determining ... Read More
The Cytochrome P450 System and Patient-Specific Pharmacotherapy
Wednesday, June 19, 2013

A portion of the more than 50 Cytochrome P450, or CYP450, enzymes are quite important from a clinical standpoint. This article looks at the role of CYP450 enzymes in drug–drug interactions and ... Read More
Binding Decisions: Designing the Optimal Custom Antibody
Friday, June 28, 2013

Commercially available antibodies may lack the functionality or specificity required for a given application, such as Western blotting or immunohistochemistry, and for this reason labs may opt for ... Read More
A Novel Sensor for Uric Acid Based on an Electropolymerization Technique
Tuesday, July 02, 2013
Youyuan Peng
A molecularly imprinted polymer (MIP) film was fabricated using an electropolymerization technique after depositing carboxyl-functionalized multiwalled carbon nanotubes (MWNTs) onto a glassy carbon ... Read More
High-Throughput Screening of Multiple Protein Complexes
Thursday, July 04, 2013
Imre Berger Maxime Chaillet Frederic Garzoni Sinyee Yau-Rose Barney Zoro
Key protein complexes are fundamental to a wide range of cellular processes and key metabolic pathways within the body. Their dysfunction is associated with a range of disease pathologies such as ... Read More
Measurement of IL-6 RNA Levels in Live Cells
Wednesday, July 10, 2013
Shobana Vaidyanathan
Infections and tissue damage lead to acute inflammatory responses, orchestrated by the release of cytokines from macrophages and mast cells, as well as endothelium and tumor cells. Tumor necrosis ... Read More
Happy 45th Anniversary, American Laboratory!
Tuesday, July 16, 2013

American Laboratory is celebrating 45 years of delivering key scientific coverage to laboratory professionals. Read More
Determination of Total Mercury Content and Trace Metal Analysis in Wood Materials—Part 1: ICP-OES Using Mercury Cold Vapor
Tuesday, August 06, 2013
Heidi Fleischer Kerstin Thurow
The transition metal mercury has many exciting properties; for example, it is the only known metal that is liquid at room temperature, hence its use in thermometers. Other common applications are in ... Read More
High-Resolution Separation of Nucleic Acid Fragments
Wednesday, August 07, 2013
Carola Schade
The invention of the polymerase chain reaction (PCR) in the early 1980s revolutionized molecular biology and molecular medicine. Since then, PCR has developed into a widely used technique for ... Read More
A Capillary Gel Electrophoresis Fluorescence System for Antibody–Antigen Interactions
Wednesday, August 07, 2013
Shou-Kuan Tsai Ming-Jhy Hseu Varoujan D. Amirkhanian
Since its inception more than 20 years ago, slab gel-based electrophoresis technology has been used routinely for the analysis of biomolecules (i.e., DNA, proteins, and carbohydrates). However, use of... Read More
In Situ Electrochemical SPM for Energy and Corrosion Studies
Wednesday, August 07, 2013
Shijie Wu
Renewable energy technologies such as solar and wind have been gaining momentum due to a growing awareness of climate change and limited resources (e.g., fossil oils) as well as greater support from ... Read More
Direct Improvement With Direct Dilution
Friday, August 09, 2013
Joe Olechno Sean Ekins Antony J. Williams Mark Fischer-Colbrie
The advent of a new technology often brings far-reaching benefits well beyond the initial perception of its key advantages. Acoustic dispensing, for example, with the ability to have highly ... Read More
Point-of-Care Tests for the Blood Lipid Profile
Wednesday, August 28, 2013

Blood lipid levels must be assessed to monitor for risk of cardiovascular disease, or CVD. The most critical lipids for risk determination are cholesterol, low-density lipoprotein (LDL), high-density ... Read More
Multiparameter Pluripotency Analysis Using Magnetic Bead Assays
Wednesday, August 28, 2013
Nikolai Stankiewicz Thomas Siegl Frederick Wiese
For the application of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) to regenerative medicine and drug discovery research, it is crucial to characterize the identity ... Read More
Rapid Characterization of Large Areas of Graphene Using Raman Spectroscopy
Thursday, August 29, 2013
Tim Batten
Raman spectroscopy probes the vibrations (phonons) in a material. These phonons are characteristic of a given material and allow its chemical and structural properties to be investigated. Read More
Dynamic Light Scattering (DLS) Microrheology: Taking Rheological Characterization to Extremes
Thursday, August 29, 2013
Steve Carrington
Microrheology is a relatively new and developing analytical methodology of growing interest to those working at the forefront of rheological characterization. The term “microrheology” is used to ... Read More
Determination of Total Mercury Content in Wood Materials, Part 2: ICP-MS—A Multielement Method
Thursday, August 29, 2013
Heidi Fleischer Kerstin Thurow
Even in minor concentrations, elemental mercury and inorganic and organic bound mercury can have harmful biological effects on organisms. Mercury and other toxic elements can enter the environment ... Read More