The accuracy and precision of pipetting can have a significant impact on downstream applications, and depending on the degree of error, poor pipetting practices can lead to inaccurate results and failed experiments. While necessary for many labs, manual pipetting carries a higher risk of error and contamination than automated liquid handling, and also raises concern about repetitive strain injury (RSI) for regular pipette users. However, routine manual pipetting can still be accurate, precise and safe by avoiding common mistakes and following best practices. Here are some of the “do’s” and “don’ts” of pipette operation to increase your confidence in your technique and results, and prevent RSI from repetitive pipetting tasks.
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