by Michelle Olson, Team Leader, Research and Develompent, Pall Biotech
There are many variables to consider for adeno-associated virus (AAV) upstream bioprocess development. These may include cell line selection and development, plasmid construction, growth mode (suspension versus adherent), transfection type and conditions, production phase, media type, and harvest methods. In addition, constraints such as scalability between benchtop and manufacturing-scale bioreactors should also be considered. Within each step there are many ways to optimize and fine-tune your process. In this article we will dive a little deeper into some of these areas to explore what AAV upstream bioprocess development may look like from a research and development point of view.
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