Peak distortions are a common and frustrating problem when performing chromatographic experiments, and peak tailing in high-performance liquid chromatography (HPLC) is one of the most frequent distortions that can occur. One of the challenging aspects of tailing peaks is that they can be caused by a range of different factors, so accurately diagnosing the problem as well as mitigating risks across your entire process are necessary to prevent further distortions. If your HPLC peaks look more like skateboard ramps than the nice Gaussian curve we all like to see, follow these tips to help identify the root of the problem and restore symmetry to your chromatograms.
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