Figure 1: Lung tissue imaged on a SLIDEVIEW™ VS200 at 20X, stained with an Ultivue PD-L1 kit multiplex kit; Dapi: Nuclear Counterstain, FITC: CD8, TRITC: CD68, Cy5: PD-L1, Cy7: panCK. Image data courtesy of Ultivue, Inc.
by David Bryce, Applications Specialist, Life Science, Evident
Whole slide scanning has emerged as an invaluable tool in a range of research and clinical applications in recent years. Given the large areas that must be covered, typical approaches involve scanning slides at low magnification (10x–20x) and have been limited in application to the detection and analysis of objects resolvable at these magnifications.
Recently, numerous improvements have been made that make high-resolution slide scanning possible. However, several tradeoffs exist when considering low- versus high-resolution scanning. In comparison, low-resolution imaging—here defined as images collected at 2x–20x magnification—provides high acquisition rates (especially for large area samples) and a macro-scale overview of features or structures, while keeping file sizes small and data volumes manageable.
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