by Josien Levenga and Andrew Riching, PerkinElmer’s Horizon Discovery
From RNA interference (RNAi) to CRISPR, there are several methods that researchers can use to manipulate gene function, each with its own strengths and weaknesses. Orthogonal validation—the synergistic use of different methods—makes genetic perturbation studies more robust. Utilizing complementary methods, including RNAi and CRISPR-knockout, -interference and -activation, enables researchers to have confidence in their results.
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