Analysis of Environmental Contamination in the Susquehanna River
Frank L. Dorman, PhD
Monday, June 24, 2019
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Chapters
Introduction
Dorman Research Group 2018-2019
Large difference in our Environment!
Humans are impacted in a wide variety of ways
The Exposome
Environmental exposure challenges
Can We Proactively Discover Exposome Issues?
What does all this have to do with fish?
Who cares about contamination in the Susquehanna River?
The Fish Exposome
Smallmouth Bass macro- and microscopic disease
Bass are indicators for the Chesapeake Bay Watershed
Globally, other fish have similar problems
Method Development: The Donald Rumsfeld School of Compound Identification
What should we investigate?
Let's Get Started: Method Development
Sample Preparation
QuEChERS: small volume, high throughput
Sample Preparation: QuEChERS-type Extraction
Instrumental Method - Needs
Instrumental Method - initial analysis
Instrumental Method - targets
Samples evaluated in this presentation
Blank matrix (Salmon muscle) tic's
Issue? Matrix and control fish are VERY different
Instrumental Method - Sample Prep decision
Matrix effect: 64 target compounds, salmon muscle tissue
Instrumental Method - performance
Total ion chromatogram, sample extracts
Data Processing - tic subtractions (effected-control)
Data Processing - target compounds
Hexa-PCB, GCxGC-BT 4D, electron ionization
Nona-PCB, GCxGC-BT 4D, electron ionization
Penta-PCB, GCxGC-BT 4D, electron ionization
PCA of top 250 features based on Fischer ratios
PCA of top 50 features based on Fischer ratios
So What
The Solutions? Known Unknowns: GCxGC-HRT
Instrumental Parameters
Analysis of Sample EF-004, EI-HRT+ 4D
EF-004, EI-HRT+ 4D: Representative Compounds
EF-004, EI-HRT+ 4D: Mass Spectra
EF-004, EI-HRT+ 4D: TIC & AIC of Selected Pollutants
EF-004, EI-HRT+ 4D: Pollutants
EF-004, EI-HRT+ 4D: Mass Spectra
Instrumental Parameters
GCxGC TIC: NCI-HRT+ 4D
EF-004, EI-HRT+ 4D Comparison
EF-004, EI-HRT+ 4D & NCI-HRT+ 4D: PCBs
EF-004, NCI-HRT+ 4D: Mass Spectra
EF-004, NCI-HRT+ 4D: Chlorinated Unknowns
EF-004, NCI-HRT+ 4D: Brominated Unknowns
Effected versus Control NCI-HRT+ 4D: PCBs
Summary
Acknowledgements
Thank you
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