Biologists are increasingly turning to fluorescence as their contrast method of choice. In addition to finding application in materials science, medicine, pharma, and semiconductors, fluorescence is also expanding into the fields of optogenetics, with stereo fluorescence on the rise. This article points out key considerations for selecting a fluorescence microscopy system, and identifies system components that need to be optimized in order to maximize output: sample, wavelength, detector, optics, and light source.
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